解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::During the course of systematic sequence tag analysis of clones isolated from an adult testis cDNA library, clones 296 and 576 were found to detect 71-74% sequence identity to the guinea pig sperm surface protein PH-20. This surface protein is involved in sperm-egg adhesion in the guinea pig. Nucleotide sequence for 1...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.9931
更新日期:1995-10-10 00:00:00
abstract::We employed the polymerase chain reaction and genomic DNA library screening to clone novel human genes, GPR9 and GPR10, and a rat gene, GPR14. GPR9, GPR10, and GPR14 each encode G protein-coupled receptors. GPR10 and GPR14 are intronless within their coding regions, while GPR9 contains at least one intron. The recepto...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.9996
更新日期:1995-09-20 00:00:00
abstract::A bacterial artificial chromosome (BAC) library has been constructed for use in bovine genome mapping using constructed for use in bovine genome mapping using the pBeloBAC11 vector. Currently, the library consists of 23,040 clones, which achieves a 70% probability (P=0.70) of the library containing a specific unique D...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.9986
更新日期:1995-09-20 00:00:00
abstract::We have constructed two YAC contigs in the Xp11.23-p11.22 interval of the human X chromosome, a region that was previously poorly characterized. One contig, of at least 1.4 Mb, links the pseudogene OATL1 to the genes GATA1, TFE3, and SYP and also contains loci implicated in Wiskott-Aldrich syndrome and synovial sarcom...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.9976
更新日期:1995-09-20 00:00:00
abstract::DNA hybridization was used to isolate a 2.04-kb cDNA encoding carboxyl ester lipase (CEL) from a mouse lactating mammary gland, lambda gt10 cDNA library. The cDNA sequence translated into a protein of 599 amino acids, including 20 amino acids of a putative signal peptide. Comparison of the deduced amino acid sequence ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1221
更新日期:1995-09-01 00:00:00
abstract::PCR primers specific to the human liver fructose-1,6-bisphosphatase (FBP) gene were designed and used to isolate a cosmid clone. Physical mapping of the FBP cosmid by FISH, and genetic mapping of an associated GA repeat polymorphism (PIC = 0.35), located the liver FBP gene to chromosome 9q22.3 with no recombination be...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1230
更新日期:1995-09-01 00:00:00
abstract::The mouse protein kinase CK2 beta subunit gene (Csnk2b) is composed of seven exons contained within 7874 bp. The exon and intron lengths extend from 76 to 321 and 111 to 1272 bp, respectively. The lengths of the murine coding exons correspond exactly to the lengths of the exons in the human CK2 beta gene. Both genes c...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1239
更新日期:1995-09-01 00:00:00
abstract::Iduronate-2-sulfatase (IDS) is involved in the degradation of heparan sulfate and dermatan sulfate in the lysosomes, and a deficiency in this enzyme results in Hunter syndrome. A 2.3-kb cDNA clone that contains the entire coding sequence of IDS has previously been reported. Here we describe the identification of a 1.4...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1249
更新日期:1995-09-01 00:00:00
abstract::We have characterized 117 cDNAs isolated by direct cDNA selection using pools of human chromosome 12p cosmids. Sequencing revealed that 41 clones did overlap with other cDNAs. Of the remaining 76 cDNA sequences, 11 matched previously identified human chromosome 12p genes and 3 matched previously determined cDNA sequen...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1213
更新日期:1995-09-01 00:00:00
abstract::The transcriptional organization of the region of the mouse X chromosome between the G6pd and the Fln1 genes was studied in detail, and it was compared with the syntenic region of the human chromosome. A cosmid contig of 250 kb was constructed by screening mouse cosmid libraries with probes for human genes and with wh...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1164
更新日期:1995-08-10 00:00:00
abstract::Mitochondrial intermediate peptidase (MIP) is a component of the mitochondrial protein import machinery required for maturation of nuclear-encoded precursor proteins targeted to the mitochondrial matrix or inner membrane. We previously characterized this enzyme in rat (RMIP) and Saccharomyces cerevisiae (YMIP) and sho...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1174
更新日期:1995-08-10 00:00:00
abstract::Expression of p107, a protein with homology to the retinoblastoma tumor suppressor (pRB), was monitored during murine development. Northern blot tissue surveys identified two transcripts of 4.9 and 2.4 kb that hybridized to a p107 cDNA clone. Expression of both transcripts was detected in fetal tissues, with particula...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1184
更新日期:1995-08-10 00:00:00
abstract::Cyclin-dependent kinase 5 (Cdk5) is predominantly expressed in neurons. In vitro, Cdk5 purified from the nervous tissue phosphorylates both high-molecular-weight neurofilament and microtubule-associated tau. The mouse gene encoding Cdk5 (Cdk5) was found to be 5 kb in length and divided into 12 exons. All of the exon-i...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1194
更新日期:1995-08-10 00:00:00
abstract::Microcell hybrids containing various regions of human chromosome 8 were formed by microcell-mediated transfer of neo-tagged chromosome 8 into the cells derived from severe combined immunodeficiency (SCID) mouse. Thus, 110 cosmid markers were isolated from SV40-transformed SCID fibroblast cell line (SCVA) containing a ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1125
更新日期:1995-07-20 00:00:00
abstract::A comparison of the murine and human junB loci reveals nine regions of distal 5'- and 3'-flanking DNA that exhibit greater than 72% sequence identity. A large fraction (over 50%) of the junB locus is contained in these flanking evolutionarily conserved sequences (FECS), which may be required for effecting the proper t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1135
更新日期:1995-07-20 00:00:00
abstract::A novel gene, TEGT (testis enhanced gene transcript), has been identified in humans. It does not belong to any known gene family of vertebrates. The deduced amino acid sequence of the gene and a bacterial protein of unknown function show low but significant homology and very similar hydrophobicity profiles. Two differ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1145
更新日期:1995-07-20 00:00:00
abstract::DNA primase is an essential replication protein that catalyzes the synthesis of oligoribonucleotide primers. DNA primase, consisting of two subunits (p49 and p58), plays a key role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis. We mapped the locations of ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1155
更新日期:1995-07-20 00:00:00
abstract::The human MAGE1 gene directs the expression of an antigen recognized on a melanoma by autologous cytolytic T lymphocytes. MAGE1 belongs to a family of genes that are expressed in a number of tumors of various histological types but not in normal tissues except testis. The MAGE genes are arranged in two groups that are...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1108
更新日期:1995-07-01 00:00:00
abstract::The single functional mouse gene for MIF (macrophage migration inhibitory factor) has been cloned from a P1 library, and its exon/intron structure determined and shown to resemble that of the human gene. The gene was mapped to chromosome 10 using two multilocus crosses between laboratory strains and either Mus musculu...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1070
更新日期:1995-06-10 00:00:00
abstract::A human corneal fibroblast cDNA library was screened with a bovine lumican cDNA probe to obtain three clones. Sequencing of the longest clone (1.75 kb) yielded an open reading frame of 1014 bp coding for a 338-amino-acid core protein. Amino acid sequencing of a tryptic peptide resulted in a 9-amino-acid match with the...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1080
更新日期:1995-06-10 00:00:00
abstract::The Alu repeat sequence is estimated to account for 5% of human genomic DNA. The precise relationship of Alu sequences to human fully spliced cDNA has yet to be determined, although many new protocols for cloning cDNAs either depend on the presence of Alus or--more usually--rely on their absence in a population of mes...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1090
更新日期:1995-06-10 00:00:00
abstract::We have isolated and chromosomally fine-mapped 16 novel genes belonging to the human zinc finger Krüppel family (ZNF131-140, 142, 143, 148, 151, 154, and 155), including 1 of the GLI type (ZNF143) and 3 containing a KRAB (Krüppel-associated box) segment (ZNF133, 136, and 140). Based on their map position, several of t...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1040
更新日期:1995-05-20 00:00:00
abstract::The promoter/5' flank sequence, cDNA sequence, and exon/intron structures of the human thrombospondin 3 (THBS3) gene have been determined. THBS3 cDNA clones were obtained by PCR amplification of human fetal lung cDNA using THBS3-specific primers. Analysis of cDNA and genomic sequences showed the THBS3 gene to be compo...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1050
更新日期:1995-05-20 00:00:00
abstract::The frizzled (fz) locus in Drosophila is required for the transmission of polarity signals across the plasma membrane in epidermal cells, as well as to their neighboring cells in the developing wing. The identification of a tissue polarity gene from the fz locus in Drosophila melanogaster has been reported. The fz gen...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1060
更新日期:1995-05-20 00:00:00
abstract::We describe the isolation and characterization of a human gene (CLCN3) and its murine homologue (Clcn3) sharing significant sequence and structural similarities with all previously identified members of the voltage-gated chloride channel (ClC) family. This gene is expressed primarily in tissues derived from neuroectod...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1015
更新日期:1995-05-01 00:00:00
abstract::Protease nexin I (PNI) is the most important physiologic regulator of alpha-thrombin in tissues. PNI is highly expressed and developmentally regulated in the nervous system where it is concentrated at neuromuscular junctions and also central synapses in the hippocampus and striatum. Approximately 10% of identified pro...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1006/geno.1995.1025
更新日期:1995-05-01 00:00:00
abstract::A genetic basis for neural tube defects (NTD) is rarely doubted, but the genes involved have not yet been identified. This is partly due to a lack of suitable families on which to perform linkage analysis. An alternative approach is to use the many mouse genes that cause NTD as a means of isolating their human homolog...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80165-i
更新日期:1995-04-10 00:00:00
abstract::Uroporphyrinogen-III synthase (URO-S; EC 4.2.1.75), the fourth enzyme in the heme biosynthetic pathway, is responsible for the conversion of hydroxymethylbilane to the cyclic tetrapyrrole, uroporphyrinogen III. The deficient activity of URO-S is the enzymatic defect in congenital erythropoietic porphyria (CEP), an aut...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80175-l
更新日期:1995-04-10 00:00:00
abstract::Core binding factor (CBF) is a heterodimeric transcription factor composed of two distinct subunits. The monomeric beta subunit is ubiquitously expressed, whereas expression of the three alpha subunits isolated previously seems to be restricted mainly to hematopoietic tissues. To isolate additional alpha genes, degene...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80185-o
更新日期:1995-04-10 00:00:00
abstract::Homozygous transgenic mice from line A4 have an early-onset progressive neuromuscular disorder characterized by paralysis of the rear limbs, muscle atrophy, and lethality by 4 weeks of age. The transgene insertion site was mapped to distal chromosome 15 close to the locus motor endplate disease (med). The sequence of ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80198-u
更新日期:1995-03-20 00:00:00
abstract::The major component of the vascular and plaque amyloid deposits in Alzheimer disease is the amyloid beta peptide (A beta). A second intrinsic component of amyloid, the NAC (non-A beta component of amyloid) peptide, has recently been identified, and its precursor protein was named NACP. A computer homology search allow...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80208-4
更新日期:1995-03-20 00:00:00
abstract::Proteins with seven transmembrane segments (7TM) define a superfamily of receptors (7TM receptors) sharing the same topology: an extracellular N-terminus, three extramembranous loops on either side of the plasma membrane, and a cytoplasmic C-terminal tail. Upon ligand binding, cytoplasmic portions of the activated rec...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80218-b
更新日期:1995-03-20 00:00:00
abstract::We report the isolation of cDNA clones for the mouse alpha 7 neuronal nicotinic acetylcholine receptor subunit (gene symbol Acra7), the only nicotinic receptor subunit known to bind alpha-bungarotoxin in mammalian brain. This gene may have relevance to nicotine sensitivity and to some electrophysiologic findings in sc...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80228-e
更新日期:1995-03-20 00:00:00
abstract::The genes for three subtilisin/kexin-like proprotein convertases, PC4, PC5, and PACE4, were mapped in the mouse by RFLP analysis of a DNA panel from a (C57BL/6JEi x SPRET/Ei)F1 x SPRET/Ei backcross. The chromosomal locations of the human homologs were determined by Southern blot analysis of a DNA panel from human-rode...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80090-9
更新日期:1995-03-01 00:00:00
abstract::Physical maps can be constructed by "fingerprinting" a large number of random clones and inferring overlap between clones when the fingerprints are sufficiently similar. E. Lander and M. Waterman (Genomics 2: 231-239, 1988) gave a mathematical analysis of such mapping strategies. The analysis is useful for comparing v...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80086-2
更新日期:1995-03-01 00:00:00
abstract::A monoclonal antibody, 170A1, which recognizes a nucleolar peptide of molecular weight 90,000, was raised. The protein was conserved among various vertebrates. To characterize the antigen, we screened a human fetal liver expression library using the monoclonal antibody as a probe. Molecular analyses of immunopositive ...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80008-a
更新日期:1995-02-10 00:00:00
abstract::Receptors for cholcystokinin (CCK) can be pharmacologically classified into at least two distinct subtypes, CCKAR and CCKBR. In an effort to determine whether the CCKA and CCKB receptors may be associated with certain CNS or gastrointestinal diseases, we have localized and compared the human and mouse chromosomal loci...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80018-h
更新日期:1995-02-10 00:00:00
abstract::The intron-containing genes encoding rat and human ribosomal protein L19 (RPL19) have been cloned. The DNA sequences of the entire rat RPL19 gene and the 5' end of the human RPL19 gene have been determined. Sequence comparison of corresponding regions of the two genes reveals a striking interspecies homology in the 5'...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80036-l
更新日期:1995-01-20 00:00:00
abstract::The acyl-CoA binding protein (ACBP) and the diazepam binding inhibitor (DBI) or endozepine are independent isolates of a single 86-amino-acid, 10-kDa protein. ACBP/DBI is highly conserved between species and has been identified in several diverse organisms, including human, cow, rat, frog, duck, insects, plants, and y...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80047-p
更新日期:1995-01-20 00:00:00
abstract::This communication describes a software tool that enables one to simulate large-scale regional mapping using an ordered shotgun sequencing approach. The analysis routines that are provided yield an estimate of the depth of coverage of the physical map, the largest contig formed, and the number of gaps remaining at any...
journal_title:Genomics
pub_type: 杂志文章
doi:10.1016/0888-7543(95)80057-s
更新日期:1995-01-20 00:00:00